Scallop shells as biosorbents for water remediation from heavy metals: Contributions and mechanism of shell components in the adsorption of cadmium from aqueous matrix.

To ascertain their potential for heavy metal pollution remedy, we studied the adsorption mechanism of cadmium onto scallop shells and the interactions between the heavy metal and the shell matrix. Intact shells were used to investigate the uptake and diffusion of the metal contaminant onto the shell carbonatic layers, as well as to evaluate the distribution of major and trace elements in the matrix. LA-ICPMS measurements demonstrate that Cd is adsorbed on a very thin layer on the inner and outer surfaces of the shell. Structural and thermal analyses showed the presence of 9 wt.-% of a CdCO3 phase indicating that the adsorption is mainly a superficial process which involves different processes, including ion exchange of Ca by Cd. In addition, organic components of the shell could contribute to adsorption as highlighted by different metal uptake observed for shells with different colours. In particular, darker shells appeared to adsorb more contaminant than the white ones. The contribution of the organic shell components on the adsorption of heavy metals was also highlighted by the element bulk content which showed higher concentrations of different metals in the darker specimen. Raman spectroscopy allowed to identify the pigments as carotenoids, confirmed by XRD measurements which highlighted the presence of astaxanthin phases. The results presented here provide new insights into the Cd adsorption mechanism highlighting the important contribution given by the organic components present in the biogenic carbonate matrix. Furthermore, the high efficiency of Cd removal from water by scallop shells, supported by adsorption kinetic and isotherm studies, has been demonstrated.

In Vitro Fossilization for High Spatial Resolution Quantification of Elements in Plant-Tissue Using LA-ICP-TOFMS.

Laser ablation in combination with an inductively coupled plasma time-of-flight mass spectrometer (LA-ICP-TOFMS) is an upcoming method for rapid quantitative element mapping of various samples. While widespread in geological applications, quantification of elements in biotissues remains challenging. In this study, a proof-of-concept sample preparation method is presented in which plant-tissues are fossilized in order to solidify the complex biotissue matrix into a mineral-like matrix. This process enables quantification of elements by using silicone as an internal standard for normalization while also providing consistent ablation processes similar to minerals to reduce image blurring. Furthermore, it allows us to generate a quantitative image of the element composition at high spatial resolution. The feasibility of the approach is demonstrated on leaves of sunflowers (Helianthus annuus), soy beans (Glycine max), and corn (Zea mays) as representatives for common crops, which were grown on both nonspiked and cadmium-spiked agricultural soil. The quantitative results achieved during imaging were validated with digestion of whole leaves followed by ICP-OES analysis. LA-ICP-TOFMS element mapping of conventionally dried samples can provide misleading trends due to the irregular ablation behavior of biotissue because high signals caused by high ablation rates are falsely interpreted as enrichment of elements. Fossilization provides the opportunity to correct such phenomena by standardization with Si as an internal standard. The method demonstrated here allows for quantitative image acquisition without time-consuming sample preparation steps by using comparatively safe chemicals. The diversity of tested samples suggests that this sample preparation method is well-suited to achieve reproducible and quantitative element maps of various plant samples.

Determination of carbon in microplastics and single cells by total consumption microdroplet ICP-TOFMS.

Recently, spICP-MS analyses of microplastics have demonstrated that the detection capabilities of ICP-MS are sufficient to determine the size and composition of such materials. However, solution nebulization or microdroplet generation limits the sizes of droplets, microparticles, or cells that can be efficiently introduced into commonly used horizontal ICP-MS configurations. Therefore, we introduced the development of a downward-pointing ICP coupled to a time-of-flight mass spectrometer (ICP-TOFMS), which enables quantitative transport of large microdroplets (diameters up to 90 µm) into the ICP. Here, we report the capabilities of downward ICP-TOFMS for the quantitative analysis of single cells and microplastic particles. For calibration of element mass amount per particle or cell, microdroplets (70 µm diameter) composed of multielement solutions were measured by ICP-TOFMS. Microplastic beads (polystyrene) and spleenocyte cells were then also embedded in microdroplets and measured by ICP-TOFMS with ion optics optimized to determine the signals from 12C+ and other isotopes of interest, including 140Ce, 153Eu, 165Ho, and 175Lu from the REE beads and 31P for the cells. The results achieved using the prototype instrument of a vertical downward-pointing ICP-TOFMS demonstrate that such a plasma configuration is well suited to analyze microplastics and single cells. For single microbead and cell analyses, the critical mass for carbon was 4.8 pg, and the mean determined carbon mass amounts were 14 and 23 pg, respectively. For the microbead analysis, the detected carbon mass corresponds to a particle diameter of 2.93 ± 0.24 µm, which is consistent with the scanning (transmission) electron microscopy-determined diameter of 2.97 ± 0.04 µm. Furthermore, in the analysis of spleenocyte cells, carbon and phosphorus masses were determined to be correlated.

A Nature-Inspired Antioxidant Strategy based on Porphyrin for Aromatic Hydrocarbon Containing Fuel Cell Membranes.

The use of hydrocarbon-based proton conducting membranes in fuel cells is currently hampered by the insufficient durability of the material in the device. Membrane aging is triggered by the presence of reactive intermediates, such as HO⋅, which attack the polymer and eventually lead to chain breakdown and membrane failure. An adequate antioxidant strategy tailored towards hydrocarbon-based ionomers is therefore imperative to improve membrane lifetime. In this work, we perform studies on reaction kinetics using pulse radiolysis and γ-radiolysis as well as fuel cell experiments to demonstrate the feasibility of increasing the stability of hydrocarbon-based membranes against oxidative attack by implementing a Nature-inspired antioxidant strategy. We found that metalated-porphyrins are suitable for damage transfer and can be used in the fuel cell membrane to reduce membrane aging with a low impact on fuel cell performance.

Colloidal CsPbX3 Nanocrystals with Thin Metal Oxide Gel Coatings.

Lead halide perovskite (LHP) nanocrystals (NCs) have gathered much attention as light-emitting materials, particularly owing to their excellent color purity, band gap tunability, high photoluminescence quantum yield (PLQY), low cost, and scalable synthesis. To enhance the stability of LHP NCs, bulky strongly bound organic ligands are commonly employed, which counteract the extraction of charge carriers from the NCs and hinder their use as photoconductive materials and photocatalysts. Replacing these ligands with a thin coating is a complex challenge due to the highly dynamic ionic lattice, which is vulnerable to the commonly employed coating precursors and solvents. In this work, we demonstrate thin (<1 nm) metal oxide gel coatings through non-hydrolytic sol-gel reactions. The coated NCs are readily dispersible and highly stable in short-chain alcohols while remaining monodisperse and exhibiting high PLQY (70-90%). We show the successful coating of NCs in a wide range of sizes (5-14 nm) and halide compositions. Alumina-gel-coated NCs were chosen for an in-depth analysis, and the versatility of the approach is demonstrated by employing zirconia- and titania-based coatings. Compact films of the alumina-gel-coated NCs exhibit electronic and excitonic coupling between the NCs, leading to two orders of magnitude longer photoluminescence lifetimes (400-700 ns) compared to NCs in solution or their organically capped counterparts. This makes these NCs highly suited for applications where charge carrier delocalization or extraction is essential for performance.

Characterizing a nitrogen microwave inductively coupled atmospheric-pressure plasma ion source for element mass spectrometry.

A high-power nitrogen-based microwave inductively coupled atmospheric-pressure plasma was coupled to a quadrupole mass spectrometer to investigate its characteristics as an ion source for element mass spectrometry. The influence of operating conditions on analyte sensitivity, plasma background, and polyatomic ion formation was investigated for conventional solution-based analysis. By varying the forward power and the nebulizer gas flow rate, the plasma background ions were found to decrease with increasing gas flow rates and decreasing operating power. Analyte ions showed different trends, which could be related to the physical-chemical properties of the elements. We could identify three groups based on the location of maximum intensity in the power vs. flow rate contour plot. Atomic ions of elements with low first ionization energy and low oxygen bond strength were found to maximize at a high nebulizer gas flow rate and lower microwave power. Elements with intermediate ionization energy and higher oxygen bond strength required higher power settings for optimum sensitivity, while elements with the highest ionization energies required the highest power and lowest gas flow rates for their optimization. The latter group showed a substantial suppression in sensitivity compared to elements of similar mass, which is considered to result from the high abundance of NO in the plasma source, whose ionization energy is close to that of these elements. Metal oxide ions were found at similar or higher abundances than in the conventional argon-based ICP and could be minimized only by using a low gas flow rate and high power settings. These general trends were also observed when the vacuum interface was modified. To change the dynamics of the supersonic expansion, different sampler cone orifice sizes and sampler-skimmer distances were investigated and the interface pressure was lowered through an additional pump. These modifications did not yield significant differences in ion transmission but lowering the interface pressure reduced the relative abundance of metal oxide ions. The limits of detection were evaluated for optimized plasma conditions and found comparable to those of an argon ICP source with the same mass spectrometer.

Lanpepsy is a novel lanthanide-binding protein involved in the lanthanide response of the obligate methylotroph Methylobacillus flagellatus.

Lanthanides were recently discovered as metals required in the active site of certain methanol dehydrogenases. Since then, the characterization of the lanthanome, that is, proteins involved in sensing, uptake, and utilization of lanthanides, has become an active field of research. Initial exploration of the response to lanthanides in methylotrophs has revealed that the lanthanome is not conserved and that multiple mechanisms for lanthanide utilization must exist. Here, we investigated the lanthanome in the obligate model methylotroph Methylobacillus flagellatus. We used a proteomic approach to analyze differentially regulated proteins in the presence of lanthanum. While multiple known proteins showed induction upon growth in the presence of lanthanum (Xox proteins, TonB-dependent receptor), we also identified several novel proteins not previously associated with lanthanide utilization. Among these was Mfla_0908, a periplasmic 19 kDa protein without functional annotation. The protein comprises two characteristic PepSY domains, which is why we termed the protein lanpepsy (LanP). Based on bioinformatic analysis, we speculated that LanP could be involved in lanthanide binding. Using dye competition assays, quantification of protein-bound lanthanides by inductively coupled plasma mass spectrometry, as well as isothermal titration calorimetry, we demonstrated the presence of multiple lanthanide binding sites that showed selectivity over the chemically similar calcium ion. LanP thus represents the first member of the PepSY family that binds lanthanides. Although the physiological role of LanP is still unclear, its identification is of interest for applications toward the sustainable purification and separation of rare-earth elements.

Detection of magnetic iron nanoparticles by single-particle ICP-TOFMS: case study for a magnetic filtration medical device.

Nanoparticles are increasingly used in medical products and devices. Their properties are critical for such applications, as particle characteristics determine their interaction with the biological system, and, therefore, the performance and safety of the final product. Among the most important nanoparticle characteristics and parameters are particle mass distribution, composition, total particle mass, and number concentration. In this study, we utilize single-particle inductively coupled plasma time-of-flight mass spectrometry (spICP-TOFMS) for the characterization of inorganic nanoparticles in complex biological fluids. We report online microdroplet calibration for reference-nanomaterial-free and matrix-matched calibration of carbon-coated iron carbide nanoparticles (C/Fe3C NPs). As a case study, we analyze C/Fe3C NPs designed for targeted blood purification. Through the analysis of NP mass distributions, we study the effect of the NP surface modification on aggregation of C/Fe3C NPs in whole blood. We also demonstrate the efficiency of removal of coated C/Fe3C NP from saline by magnetically enhanced filters. Magnetic filtering is shown to reduce the mass concentration of detectable C/Fe3C NPs by 99.99 ± 0.01% in water.

Quantification and Clustering of Inorganic Nanoparticles in Wastewater Treatment Plants across Switzerland.

Single particle Inductively Coupled Plasma Time-of-Flight Mass Spectrometry (sp-ICP-TOFMS), in combination with online microdroplet calibration, allows the determination of particle number concentrations (PNCs) and the masses of elements in individual particles. Because sp-ICP-TOFMS analyses of environmental samples produce rich datasets composed of both single-metal nanoparticles (smNPs) and many types of multimetal NPs (mmNPs), interpretation of these data is well suited to automated analysis schemes. Here, we present a data analysis approach that includes automatic particle detection and elemental mass determinations based on online microdroplet calibration, and unsupervised clustering analysis of mmNPs to identify unique classes of NPs based on their element compositions. To demonstrate the potential of our approach, we analyzed wastewater samples collected from the influent and effluent of five wastewater treatment plants (WWTPs) across Switzerland. We determined elemental masses in individual NPs, as well as PNCs, to estimate the NP removal efficiencies of the individual WWTPs. Through hierarchical clustering, we identified NP classes conserved across all WWTPs; the most abundant particle types were those rich in Ce-La, Fe-Al, Ti-Zr, and Zn-Cu. In addition, we found particle types that are unique to one or a few WWTPs, which could indicate point sources of anthropogenic NPs.

LA-ICP-MS using a nitrogen plasma source.

Here we describe the first study of a nitrogen based inductively coupled plasma mass spectrometry system in conjunction with laser ablation (LA-(N2-ICP)-MS). Therefore, a microwave-sustained, inductively coupled, atmospheric-pressure plasma source was mounted onto the interface of a quadrupole ICP-MS to investigate the capabilities of such an instrument. The proof of concept study was focused on the quantification capabilities of major to trace elements. Therefore, the plasma background species under dry plasma conditions were investigated to identify the most suitable isotopes for the analysis and to describe the newly formed nitrogen plasma interferences. In addition, the instrumental drift was investigated. Selected elements in the reference materials NIST SRM 612 and BCR-2G were quantified using NIST SRM 610 as an external standard and could be determined within the uncertainty of the reference values. Finally, the limits of detection for LA-(N2-ICP)-MS and LA-(Ar-ICP)-MS were compared indicating similar or even lower LODs for most elements using LA-(N2-ICP)-MS. Therefore, a nitrogen plasma source coupled to a mass spectrometer could challenge the argon-sustained ICP-MS in element analysis by overcoming argon interferences and has the potential to reduce the plasma gas expenses significantly.

Emerging investigator series: automated single-nanoparticle quantification and classification: a holistic study of particles into and out of wastewater treatment plants in Switzerland.

Single particle inductively coupled plasma time-of-flight mass spectrometry (sp-ICP-TOFMS), in combination with online microdroplet calibration, allows for the determination of particle number concentrations (PNCs) and the amount (i.e. mass) of ICP-MS-accessible elements in individual particles. Because sp-ICP-TOFMS analyses of environmental samples produce rich datasets composed of both single-metal nanoparticles (smNPs) and many types of multi-metal NPs (mmNPs), interpretation of these data is well suited to automated analysis schemes. Here, we present a new data analysis approach that includes: 1. automatic particle detection and elemental mass determinations based on online microdroplet calibration, 2. correction of false (randomly occurring) multi-metal associations caused by measurement of coincident but distinct NPs, and 3. unsupervised clustering analysis of mmNPs to identify unique classes of NPs based on their element compositions. To demonstrate the potential of our approach, we analyzed water samples collected from the influent and effluent of five wastewater treatment plants (WWTPs) across Switzerland. We determined elemental masses in individual NPs, as well as PNCs, to estimate the NP removal efficiencies of the individual WWTPs. From WWTP samples collected at two points in time, we found an average of 90% and 94% removal efficiencies of single-metal and multi-metal NPs, respectively. Between 5% to 27% of detected NPs were multi-metal; the most abundant particle types were those rich in Ce-La, Fe-Al, Ti-Zr, and Zn-Cu. Through hierarchical clustering, we identified NP classes conserved across all WWTPs, as well as particle types that are unique to one or a few WWTPs. These uniquely occurring particle types may represent point sources of anthropogenic NPs. We describe the utility of clustering analysis of mmNPs for identifying natural, geogenic NPs, and also for the discovery of new, potentially anthropogenic, NP targets.

Lone-Pair-Induced Structural Ordering in the Mixed-Valent 0D Metal-Halides Rb23BiIII x SbIII 7-x SbV 2Cl54 (0 ≤ x ≤ 7).

Mixed-valent metal-halides containing ns2 lone pairs may exhibit intense visible absorption, while zero-dimensional (0D) ns2-based metal-chlorides are generally colorless but have demonstrated promising optoelectronic properties suitable for thermometry and radiation detection. Here, we report solvothermally synthesized mixed-valent 0D metal-halides Rb23BiIII x SbIII 7-x SbV 2Cl54 (0 ≤ x ≤ 7). Rb23SbIII 7SbV 2Cl54 crystallizes in an orthorhombic space group (Cmcm) with a unique, layered 0D structure driven by the arrangement of the 5s2 lone pairs of the SbIIICl6 octahedra. This red material is likely the true structure of a previously reported monoclinic "Rb2.67SbCl6" phase, the structure of which was not determined. Partially or fully substituting SbIII with isoelectronic BiIII yields the series Rb23BiIII x SbIII 7-x SbV 2Cl54 (0 < x ≤ 7), which exhibits a similar layered 0D structure but with additional disorder that yields a trigonal crystal system with an enantiomorphic space group (R32). Second harmonic generation of 532 nm light from a 1064 nm laser using Rb23BiIII 7SbV 2Cl54 powder confirms the noncentrosymmetry of this space group. As with the prototypical mixed-valent pnictogen halides, the visible absorption bands of the Rb23BiIII x SbIII 7-x SbV 2Cl54 family are the result of intervalent SbIII-SbV and mixed-valent BiIII-SbV charge transfer bands (CTB), with a blueshift of the absorption edge as BiIII substitution increases. No PL is observed from this family of semiconductors, but a crystal of Rb23BiIII 7SbV 2Cl54 exhibits a high resistivity of 1.0 × 1010 Ω·cm and X-ray photoconductivity with a promising µτ product of 8.0 × 10-5 cm2 s-1 V-1. The unique 0D layered structures of the Rb23BiIII x SbIII 7-x SbV 2Cl54 family highlight the versatility of the ns2 lone pair in semiconducting metal-halides, pointing the way toward new functional 0D metal-halide compounds.

Listening with Curiosity - Tracking the Acoustic Response of Portable Laser Ablation.

Nowadays, one of the methods of choice for minimal invasive sampling of solid matter is laser ablation (LA). Routine LA sampling is performed commonly in the laboratory and the amount of ablated mass can directly be monitored and analysed. By contrast laser-based sampling in the field, using a portable laser ablation system (pLA), still remains challenging concerning low-absorbing or NIR-transparent samples. The current hardware is limited in regards to photon energy and density resulting in unsteady ablation. But as the actual amount of collected mass is the major crux of on-site sampling, with this performance it is often unknown and estimates can only be made based on the experience from prior method development and the experience of the user. In the following work an easy-to-use method to monitor the amount of ablated material collected during laser-based sampling by measuring the acoustic response is presented. The pLA-system was coupled to inductively coupled plasma mass spectrometry (ICPMS) via a diffusion driven gas exchange device (GED) which allowed to monitor mass removal and acoustic response quasi-simultaneously. For the current instrumentation only actual mass removal leads to the formation of shockwaves (SW) and, thus, acoustic signals. These events can be used as indicator for executed LA events and counted on an individual basis. The intensity of acoustic signals has been shown to correlate with the LA mass, i.e., the amount of ablated material. This allows to perform re-adjustment of the laser focus during sampling for optimal ablation based on the intensity of the acoustic signal. Likewise, acoustic intensity together with counting allows the operator to make estimates about total mass sampled. Therefore, unsuccessful laser aerosol collection in the field shall become a thing of the past.

Fundamental studies on droplet throughput and the analysis of single cells using a downward-pointing ICP-time-of-flight mass spectrometer.

Capabilities of the downwardly oriented inductively coupled plasma mass spectrometer (ICP-MS) recently reported (Vonderach et al. 2021) were studied using a time-of-flight mass spectrometer (TOFMS) yielding benefits for the fast detection of short transient signals containing multi-element information. The previously reported sample inlet configuration for the analysis of microdroplets was equipped with two extra gas inlets for the supply of argon and helium, which enabled a more precise optimization of the sample introduction and operating conditions of the plasma. Furthermore, the sample supply system was operated at elevated temperatures to enhance the desolvation of the droplets prior to their introduction into the plasma. Transient droplet signals with frequencies of up to 1000 Hz were recorded for 74 µm (diameter) sized droplets. The upper detectable droplet size was limited by the droplet generator used and was measured at 93 µm (diameter). The droplets served as the transporter for biological cells so that the described setup could be used to analyze single cells. Mouse lung cells embedded into droplets were detected successfully according to their Cs droplet tracer, Ir nucleus marker, surface markers and the phosphorus content. Transient signals were recorded at a time resolution of 33 µs in order to investigate the signal structure of single droplet-cell events containing multiple elements. Signals between 200-400 µs (FW base) and ≤100 µs (FWHM) in duration were measured. To ensure that the droplet formation process did not affect the sampled cells, different types of cells were localized within the droplets using optical inspection directly after droplet formation and it was possible to observe that cells remained intact with random sampling. The results indicate that a downward-pointing ICP-MS in combination with the microdroplet-based approach can be considered as an alternative to commonly used ICP-MS systems for single cell analysis, and might be suitable for online coupling to flow cytometry.

New Orientation: A Downward-pointing Vertical Inductively Coupled Plasma Mass Spectrometer for the Analysis of Microsamples.

We present a prototype of a vertical-downward configuration of an inductively coupled plasma mass spectrometer (ICPMS) allowing the sample introduction from the top. With this novel approach to orient the ICP downward, we aim to expand the sample transport capabilities in ICPMS especially for the transport of droplets or particles with a final goal to analyze individual cells. Because of this gravity-assisted sampling approach, the transport of larger sized droplets, that is, droplets that would be difficult to transport into a horizontally oriented ICPMS, becomes possible and, furthermore, becomes independent of the droplets' size or size distribution. We demonstrate that droplets of an initial size of 70 µm can be successfully transported into the plasma at dispensing frequencies up to 1 kHz without the need for a desolvation device. In addition, we observed that the implementation of a desolvation device, that is, a gas-exchange device (GED), can improve the detection efficiencies (DEs). Compared to operating conditions that are commonly reported for ICPMS experiments, significantly different optimization parameters (radio frequency power and gas flow rates) were tested in the presented experiments here while instrument type-specific DEs were obtained.

The Ins and Outs of 14C Dating Lead White Paint for Artworks Application.

Lead white is known as one of the oldest pigments in art and can be used as a dating material. Upon production following the Stack process, the 14C isotope of atmospheric carbon dioxide is fixed in the carbonate, and its radiocarbon dating can be used as a proxy for the age of a painting. The previously reported carbonate hydrolysis protocol reaches its limitation when confronted with samples presenting a mixture of carbonates, such as lead carbonate (cerussite or hydrocerussite), calcium carbonate (calcite), and/or calcium magnesium carbonate (dolomite). Thermogravimetric analyses indicate that decomposition of lead carbonate can be achieved at 350 °C in TGA diagrams, as other mineral carbonates only decompose to carbon dioxide at temperatures above 700 °C. Thus, a thermal approach is proposed to separate the various carbonates and isolate the specific 14C signature to the lead carbonate. In practice, however, discrepancies between the measured radiocarbon ages and expected ages were observed. FTIR analyses pointed to the formation of metal carboxylates, an indicator that the organic binder is not inert and plays a role in the dating strategy. Upon drying, oxidation and hydrolysis take place leading to the formation of free fatty acids, which in turn interact with the different carbonates upon heating. Their removal was achieved by introduction of a solvent extraction step prior to the thermal treatment, which was confirmed by GC-MS analyses, and thus, the collected carbon dioxide at 350 °C results can be assigned correctly to the decomposition of the lead white pigment. The proposed procedure was furthermore verified on mixed carbonate-bearing paint samples collected from a Baroque oil painting.

Dual isotope system analysis of lead white in artworks.

Isotopic studies are gaining much interest in heritage science, as they can provide insight into a material's age and provenance. Radiocarbon (14C) dating affords a time frame for the materials being studied, thus providing a historical context, whereas the specific pattern of lead isotope ratios may be used to set geographical constraints on the source of the original materials. Both methods require invasive sampling from the object, and henceforth limits their respective application. With the focus on lead white paint (2PbCO3·Pb(OH)2), in this study we extract the time of production of the pigment from the carbonate anion by radiocarbon dating while its origin is traced by lead isotope analysis on the cation. The methodology was applied to 12 British and 8 Swiss paintings from the 18th to 20th century, with known dates and provenance. The 14C analysis of the lead white in combination with the organic binder and canvas alone places all objects between the 17th and 20th centuries, which is in agreement with their signed date, wheras the lead isotope analysis of all samples are consistent with lead ores from European deposits. In most of the cases the combined results are consistent with the art historical data and prove that isotope analysis is intrinsic to the object. This feasibility study conducted on paintings of known age demonstrates the possibility to maximize the information output from lead white paint, thus increasing the benefits of a single sampling.

Intracavitary cisplatin-fibrin chemotherapy after surgery for malignant pleural mesothelioma: A phase I trial.

OBJECTIVES: Intracavitary chemotherapy is a promising concept to improve local tumor control for malignant pleural mesothelioma with reported high morbidity rates. We have demonstrated that administration of cisplatin loaded to fibrin increased local drug concentration and reduced systemic toxicity in preclinical models. We present a phase I trial of intracavitary cisplatin-fibrin after surgical tumor resection. METHODS: A total of 12 patients (75% International Mesothelioma Interest Group stage III-IV) were treated with 4 dose levels of intracavitary cisplatin-fibrin (11-44 mg/m2 body surface area) in a dose-escalating design. Cisplatin-fibrin was sprayed on the resected surfaces after pleurectomy/decortication. Blood and tissue samples were taken to assess toxicity and pharmacokinetics. Patients were regularly followed up. RESULTS: No dose-limiting toxicity was observed. Major morbidity occurred in 4 patients (33%). The 30-day and 90-day mortality were both 0%. Of 80 adverse events, 9 were classified serious, but none of these were related to study treatment. Local cisplatin concentration in the chest wall tissue was high at all dose levels (median, 46.3 µg/g [12-133 µg/g]). In serum, median cisplatin area under the concentration time curve values were always below renal toxicity levels. The median overall survival with 95% confidence interval was 21 months (10-31 months). In 1 patient with epithelioid malignant pleural mesothelioma (International Mesothelioma Interest Group stage I), there was no sign of relapse 48 months after treatment (44 mg/m2 body surface area). CONCLUSIONS: The administration of intracavitary cisplatin-fibrin is safe with favorable pharmacokinetics. Although most patients had advanced disease, long-term outcomes are comparable to other multimodal concepts. A confirmation phase II trial is ongoing.

Uncovering modern paint forgeries by radiocarbon dating.

Art forgeries have existed since antiquity, but with the recent rapidly expanding commercialization of art, the approach to art authentication has demanded increasingly sophisticated detection schemes. So far, the most conclusive criterion in the field of counterfeit detection is the scientific proof of material anachronisms. The establishment of the earliest possible date of realization of a painting, called the terminus post quem, is based on the comparison of materials present in an artwork with information on their earliest date of discovery or production. This approach provides relative age information only and thus may fail in proving a forgery. Radiocarbon (14C) dating is an attractive alternative, as it delivers absolute ages with a definite time frame for the materials used. The method, however, is invasive and in its early days required sampling tens of grams of material. With the advent of accelerator mass spectrometry (AMS) and further development of gas ion sources (GIS), a reduction of sample size down to microgram amounts of carbon became possible, opening the possibility to date individual paint layers in artworks. Here we discuss two microsamples taken from an artwork carrying the date of 1866: a canvas fiber and a paint chip (<200 µg), each delivering a different radiocarbon response. This discrepancy uncovers the specific strategy of the forger: Dating of the organic binder delivers clear evidence of a post-1950 creation on reused canvas. This microscale 14C analysis technique is a powerful method to reveal technically complex forgery cases with hard facts at a minimal sampling impact.